There are crystals stuck to the top of the bottle of chloroform and isoamyl alcohol-phenol. What are they? The crystals are glued to the glass pieces are cloudy and are above the liquid surface. This is a brand new, unopened bottle of 25:24:1.
Phenol is a solid with a melting point of 40.5 ° C.
Chloroform and isoamyl alcohol are solvents for this solution. Phenol is released to the solution and condensed on the sides of the glass bottle.
All you have to do is mix (vortex) inside the bottle until dissolved phenol (before opening the bottle).
Posted on February 8, 2010.